Towards non-animal testing with TOX FLOW

Media.Source: ivarpel.nl
Within the TOX FLOW project we develop non-animal testing methods to study the influence of toxic substances and complex mixtures on the reproduction and development of embryos.

Objective

By means of the TOX FLOW project we want to develop animal-free testing methods to predict the influence of toxic substances on the reproduction and development of embryos.

Using animal testing to test the many different chemicals takes a lot of time and money. Companies and governments are therefore asking for reliable non-animal testing methods to be developed, that also need to be accepted by regulatory authorities in Europe (and beyond).
 

Results

We put the insights from our research into practice by means of:

  • making standard procedures available for the various testing methods; 
  • writing scientific articles and delivering presentations for researchers, companies and governments;
  • the European Regional Development Fund (ERDF), which is also intended to stimulate economic growth. There is a lot of interest for the commercial application of the results;
  • providing protocols and internships for bachelor students.

 

Duration

01 December 2018 - 31 December 2022

Approach

For this project, methods that were developed previously (the C elegans worm, zebrafish embryos and stem cells) are combined with in-vitro skin models to test the effects of complex compounds. Skin models are used to measure the exposure to these substances through the skin.

This research is important for companies that develop (chemical) products or have their products tested via animal testing methods by contract research organisations (CROs).

This research project is a follow-up to the PreDART project. DART stands for Development And Reproduction Toxicology and is also referred to as development and reproduction toxicology. Virtually all companies in the chemical industry are obliged to test their products in accordance with DART, due to the European REACH law.

 

HU researchers involved in the research

  • Cyrille Krul
    Cyrille Krul
    • Professor
    • Research groups: Innovative Testing in Life Sciences and Chemistry
  • Raymond Pieters | Professor | Research group Innovative Testing in Life Sciences & Chemistry
    Raymond Pieters
    • Professor
    • Research groups: Innovative Testing in Life Sciences and Chemistry
  • Marc Teunis | Researcher | Research group Innovative Testing in Life Sciences and Chemistry
    Marc Teunis
    • Researcher
    • Research groups: Innovative Testing in Life Sciences and Chemistry
  • Johanna Louter-van de Haar | Researcher | Research group Innovative Testing in Life Sciences and Chemistry
    Johanna Louter - van de Haar
    • Researcher
    • Research groups: Innovative Testing in Life Sciences and Chemistry
  • Marjolein Wildwater | Researcher | Research group Innovative Testing in Life Sciences and Chemistry
    Marjolein Wildwater
    • Researcher
    • Research groups: Innovative Testing in Life Sciences and Chemistry

Collaboration with knowledge partners

In addition to HU University of Applied Sciences Utrecht, our partners in this project are:
IRAS-UU
WUR
Wageningen Food Safety Research (formerly RIKILT)
PamGene
Vivaltes (a spin-out of the previous NC3R PreDART project).
The total budget of this project is 4.5 million Euro
 

Would you like to collaborate or do you have any questions?

Marjolein Wildwater | Researcher | Research group Innovative Testing in Life Sciences and Chemistry

Marjolein Wildwater

  • Researcher
  • Research group: Innovative Testing in Life Sciences and Chemistry