Adaptation of exercise-induced stress in well-trained healthy young men

Authors Marco Mensink , Raymond Pieters , Jaap Keijer , Lars Ridder , Martie Verschuren , Stefan Nierkens , Richard Bas , Kaatje Lenaerts , Renger F. Witkamp , Shirley Kartaram , Harry Wichers , Klaske van Norren , Lonneke Janssen - Duijghuijsen
Published in Experimental Physiology
Publication date 30 November 2016
Research groups Innovative Testing in Life Sciences and Chemistry
Type Article

Summary

from the article: Abstract Strenuous exercise induces different stress-related physiological changes, potentially including changes in intestinal barrier function. In the Protégé Study (ISRCTN14236739; www.isrctn.com) we determined the test-retest repeatability in responses to exercise in well-trained individuals. Eleven well-trained males (27 ± 4 years old) completed an exercise protocol that consisted of intensive cycling intervals, followed by an overnight fast and an additional 90 min cycling phase at 50% Wmax the next morning. The day before (rest), and immediately after the exercise protocol (exercise) a lactulose/rhamnose solution was ingested. Markers of energy metabolism, lactulose/rhamnose ratio, several cytokines and potential stress-related markers were measured at rest and during exercise. In addition, untargeted urine metabolite profiles were obtained. The complete procedure (Test) was repeated one week later (Retest) to assess repeatability. Metabolic effect parameters with regard to energy metabolism and urine metabolomics were similar for both the Test and Retest period, underlining comparable exercise load. Following exercise, intestinal permeability (one hour plasma lactulose/rhamnose ratio), serum interleukin-6, interleukin-10, fibroblast growth factor-21, and muscle creatine kinase levels were only significantly increased compared to rest during the first test and not when the test was repeated. Responses to strenuous exercise in well-trained young men, as indicated by intestinal markers and myokines, show adaptation in Test-Retest outcome. This might be due to a carry-over effect of the defense mechanisms triggered during the Test. This finding has implications for the design of studies aimed at evaluating physiological responses to exercise. This is an Accepted Article that has been peer-reviewed and approved for publication in the Experimental Physiology, but has yet to undergo copy-editing and proof correction

researchcomponents.publicationcontent.personslist.publicationauthors

  • Raymond Pieters | Professor | Research group Innovative Testing in Life Sciences & Chemistry
    Raymond Pieters
    • Professor
    • Research groups: Innovative Testing in Life Sciences and Chemistry
  • Shirley Kartaram | Researcher | Research group Innovative Testing in Life Sciences and Chemistry
    Shirley Kartaram
    • Researcher
    • Research groups: Innovative Testing in Life Sciences and Chemistry

Language English
Published in Experimental Physiology
Key words Exercise, test-retest, intestinal permeability, stress response

Innovative Testing in Life Sciences and Chemistry