The effect of endurance exercise on intestinal integrity in well‐trained healthy men

Authors Lonneke Janssen - Duijghuijsen, Marco Mensink, Kaatje Lenaerts, Ewa Fiedorowicz, Dorien van Dartel, Jurriaan Mes, Yvette Luiking, Harry Wichers, Renger Witkamp, Klaske van Norren, Shirley Kartaram, Anne Geijsen, Astrid van der Harst, Martie Verschuren, Leon Knippels, Raymond Pieters
Published in Physiological Reports
Publication date 2016
Research groups Innovative Testing in Life Sciences and Chemistry
Type Article


Exercise is one of the external factors associated with impairment of intestinal integrity, possibly leading to increased permeability and altered absorption. Here, we aimed to examine to what extent endurance exercise in the glycogen‐depleted state can affect intestinal permeability toward small molecules and protein‐derived peptides in relation to markers of intestinal function. Eleven well‐trained male volunteers (27 ± 4 years) ingested 40 g of casein protein and a lactulose/rhamnose (L/R) solution after an overnight fast in resting conditions (control) and after completing a dual – glycogen depletion and endurance – exercise protocol (first protocol execution). The entire procedure was repeated 1 week later (second protocol execution). Intestinal permeability was measured as L/R ratio in 5 h urine and 1 h plasma. Five‐hour urine excretion of betacasomorphin‐7 (BCM7), postprandial plasma amino acid levels, plasma fatty acid binding protein 2 (FABP‐2), serum pre‐haptoglobin 2 (preHP2), plasma glucagon‐like peptide 2 (GLP2), serum calprotectin, and dipeptidylpeptidase‐4 (DPP4) activity were studied as markers for excretion, intestinal functioning and recovery, inflammation, and BCM7 breakdown activity, respectively. BCM7 levels in urine were increased following the dual exercise protocol, in the first as well as the second protocol execution, whereas 1 h‐plasma L/R ratio was increased only following the first exercise protocol execution. FABP2, preHP2, and GLP2 were not changed after exercise, whereas calprotectin increased. Plasma citrulline levels following casein ingestion (iAUC) did not increase after exercise, as opposed to resting conditions. Endurance exercise in the glycogen depleted state resulted in a clear increase of BCM7 accumulation in urine, independent of DPP4 activity and intestinal permeability. Therefore, strenuous exercise could have an effect on the amount of food‐derived bioactive peptides crossing the epithelial barrier. The health consequence of increased passage needs more in depth studies.

On this publication contributed

  • Shirley Kartaram | Researcher | Research group Innovative Testing in Life Sciences and Chemistry
    Shirley Kartaram
    • Researcher
    • Research group: Innovative Testing in Life Sciences and Chemistry
  • Raymond Pieters | Professor | Research group Innovative Testing in Life Sciences & Chemistry
    Raymond Pieters
    • Professor
    • Research group: Innovative Testing in Life Sciences and Chemistry

Language English
Published in Physiological Reports
Year and volume 4 20
Key words dipeptidylpeptidase-4, amino acids, citrulline, exercise, intestinal permeability, betacasomorphin-7
Digital Object Identifier 10.14814/phy2.12994

Innovative Testing in Life Sciences and Chemistry