The mycotoxin deoxynivalenol facilitates allergic sensitization to whey in mice

Authors Manon van Roest , S. Braber , P. Akbari , P.de de Graaff , J.J. Smit , P.V. Jeurink , B.C. van Esch , L. Kruijssen , J. Garssen , Raymond Pieters , J. Fink-Gremmels , Marianne Bol-Schoenmakers
Published in Mucosal Immunology
Publication date 1 November 2016
Research groups Innovative Testing in Life Sciences and Chemistry
Type Article

Summary

Abstract from the article: Intestinal epithelial stress or damage may contribute to allergic sensitization against certain food antigens. Hence, the present study investigated whether impairment of intestinal barrier integrity by the mycotoxin deoxynivalenol (DON) contributes to the development of whey-induced food allergy in a murine model. C3H/HeOuJ mice, orally exposed to DON plus whey once a week for 5 consecutive weeks, showed whey-specific IgG1 and IgE in serum and an acute allergic skin response upon intradermal whey challenge, although early initiating mechanisms of sensitization in the intestine appeared to be different compared with the widely used mucosal adjuvant cholera toxin (CT). Notably, DON exposure modulated tight-junction mRNA and protein levels, and caused an early increase in IL-33, whereas CT exposure affected intestinal γδ T cells. On the other hand, both DON- and CT-sensitized mice induced a time-dependent increase in the soluble IL-33 receptor ST2 (IL-1R1) in serum, and enhanced local innate lymphoid cells type 2 cell numbers. Together, these results demonstrate that DON facilitates allergic sensitization to food proteins and that development of sensitization can be induced by different molecular mechanisms and local immune responses. Our data illustrate the possible contribution of food contaminants in allergic sensitization in humans.

researchcomponents.publicationcontent.personslist.publicationauthors

  • Raymond Pieters | Professor | Research group Innovative Testing in Life Sciences & Chemistry
    Raymond Pieters
    • Professor
    • Research groups: Innovative Testing in Life Sciences and Chemistry

Language English
Published in Mucosal Immunology
Year and volume 9 6
ISBN/ISSN URN:ISSN:1933-0219
Key words food allergy
Page range 1477-1486

Innovative Testing in Life Sciences and Chemistry