Comparison of Six Commercial ELISA Kits for Their Specificity and Sensitivity in Detecting Different Major Peanut Allergens

Authors Julie Nordlee , Aard de Jongh , Joe Baumert , Raymond Pieters , Steve L. Taylor , Stef J. Koppelman , Shyamali Jayasena , Daniëlle Fiechter , Mieke Smits
Published in Journal of agricultural and food chemistry
Publication date 17 January 2015
Research groups Innovative Testing in Life Sciences and Chemistry
Type Article

Summary

Author supplied: "Six commercial peanut enzyme-linked immunosorbent assay kits were assessed for their ability to recover peanut from the standard reference material 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3, and Ara h 6. The percentage recovery of peanut from peanut butter differed across different kits as well as at different sample concentrations. The highest recovery was observed with the Romer and R-Biopharm kits, while four other kits were found to underestimate the protein content of the reference peanut butter samples. Five of the kits were most sensitive in detecting Ara h 3 followed by Ara h 1, while hardly recognizing Ara h 2 and Ara h 6. The other kit showed the highest sensitivity to Ara h 2 and Ara h 6, while Ara h 1 and Ara h 3 were poorly recognized. Although Ara h 2 and Ara h 6 are known to be heat stable and more potent allergens, antisera specific to any of these four peanut proteins/allergens may serve as good markers for the detection of peanut residues."

researchcomponents.publicationcontent.personslist.publicationauthors

  • Raymond Pieters | Professor | Research group Innovative Testing in Life Sciences & Chemistry
    Raymond Pieters
    • Professor
    • Research groups: Innovative Testing in Life Sciences and Chemistry
  • Mieke Smits | Researcher | Research group Innovative Testing in Life Sciences and Chemistry
    Mieke Smits
    • Researcher
    • Research groups: Innovative Testing in Life Sciences and Chemistry

Language English
Published in Journal of agricultural and food chemistry
Year and volume 63 6
ISBN/ISSN URN:ISSN:0021-8561
Key words Peanuts, Allergies, Testing
Page range 1849-1855

Innovative Testing in Life Sciences and Chemistry